WebMar 27, 2024 · Then, the culture was divided into three aliquot parts and spread on three LB plates supplemented with 1 mM IPTG, 40 μg/mL X-gal, and 25 μg/mL kanamycin, 50 μg/mL spectinomycin, or 15 μg/mL tetracycline as needed. The plates were incubated overnight at 37 °C. The transformation experiment was conducted thrice for replication. WebIPTG (isopropylthiogalactoside) is an inducer of the lac operon in bacteria, which is frequently used in cloning as a component of a recombinant plasmid. When used with X …
X-Gal GoldBio
WebApr 4, 2024 · (D) The E. coli K-12 BW25113 WT and ∆ lon strains harboring the plasmid pCA24N-NHis-yefM were induced with 0.5 mM IPTG for 30 min, and 1% spectinomycin (100 µg/mL) was added to the strains to activate a stress response. We collected equivalent quantities of cells at 0 min, 30 min, 60 min and 120 min and then ran Tricine-SDS-PAGE … WebPreparation of X-Gal/IPTG LB Agar Plates for Blue/White Colony Screening For individual LB (Luria Broth) agar plates: 1. Pour sterile warm LB agar (about 25 mL) into a Petri dish. 2. … ear suction item number
Plasmids 101: Blue-white Screening - Addgene
WebSep 13, 2024 · The present invention provides a method for manufacturing an artificial polypeptide fiber, the method comprising: a step for bundling a plurality of raw material fibers containing an artificial polypeptide; and a step for bringing an obtained bundle of raw material fibers into contact with a composition containing a binder and an enzyme. WebProduct Description. 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate. X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. WebIPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is … ear suction waikanae